The Prairie Siding Bridge on Jacob Rd. west of Chatham will be closed on Tuesday between River View Line and Grand River Line.
The closure takes place from 8am to 5pm to continue the environmental cleanup after a tanker rolled over May 9 and spilled 35,000 L of diesel in a drainage ditch.
The fuel didn't reach the Thames River.
Damage, including the cost of the cleanup, is estimated at more than $250,000.
Drivers needing to cross the Thames River on Tuesday are being asked to use the Parry Bridge on Keil Dr.