The Ministry of Natural Resources laid 35 charges and issued 27 warnings during an enforcement blitz on the Detroit River.
The long weekend blitz also took place at Inner Long Point Bay south of Port Rowan.
Conservation officers checked 749 anglers and laid charges and warnings for fishing without a licence, fishing in a sanctuary, possessing live invasive species, and using more than the permitted number of lines.
They also educated the public about invasive species and the negative effect they can have on the biodiversity in the Detroit River and surrounding waters.